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KMID : 0390119920320010001
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Journal of Pusan Medical College
1992 Volume.32 No. 1 p.1 ~ p.14
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Characterization of Calcium Current in Smooth Muscle Cell of Rabbit Colon
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장영록
정동근/전진섭/김용근/이상호
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Abstract
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Whole cell patch-clamp technique was used to investigate the characteristics of Ca2+ currents in the enzymatically isolated colonic smooth muscle cells of rabbit. Isolated cell contracted reversibly in response to high K+ (150 mM). The
current-voltage
relationship of Ca2+ current presented the bell shape. The amplitude of Ca2+ current was dependent to extracellular Ca2+ concentration up to 5 mM. The voltage of half inactivation(V1/2) of the peak component obtained from the curve fit was -39.5
mV
and
it was suggested a presence of window current. Inactivation of Ca2+ current could be well fitted by single exponential decay. Various inorganic Ca2+ channel blockers suppressed Ca2+ current and their potencies shows the following order ;
Cb〉Co〉Ni〉Mn.
Nifedipine (1μM) decreased Ca2+ current, whereas BAY K 8644 (1μM) increased Ca2+ current. The Ba2+ was more permeable than Ca2+ through Ca2+ channel.
These results suggest that charge carrier of inward current is Ca2+ through L type Ca2+ channels in smooth muscle cell of rabbit colon.
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KEYWORD
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